Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension

Diaspro, Alberto

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Details

Published by: Springer

Publication Date: 2010-11-30

Format: Hardcover

ISBN-13: 9783642151743

DOI: 10.1007/978-3-642-15175-0

Dimensions: 235cm x155cm

Pages: 244