This Open Access book provides a focused translational roadmap on mesenchymal stem cells (MSCs) and MSC-derived extracellular vesicles (MSC-EVs), covering their biology, molecular cargo, isolation and characterization, mechanisms of action, preclinical evidence, clinical translation, and GMP/regulatory considerations. The book emphasizes the current shift from cell-based therapies toward cell-free therapeutic platforms and critically evaluates the opportunities and limitations of MSC-EV-based interventions in regenerative medicine. The intended audience includes stem cell and extracellular vesicle researchers, translational scientists, and investigator-clinicians working in regenerative medicine. The book can also serve as a useful resource for clinician-scientists, biotechnology developers, regulatory professionals, and graduate students in stem cell biology, translational therapeutics, and biomedical sciences.

Prof. Dr. Nadiar M. Mussin is a researcher in regenerative medicine and translational biomedical sciences with substantial expertise in mesenchymal stem cells, extracellular vesicles, and experimental therapeutic strategies. His work integrates laboratory-based investigations with clinical and translational applications, focusing on the development and evaluation of innovative regenerative approaches. He has contributed to nationally and internationally funded research projects in the fields of stem cell therapy and extracellular vesicle–based interventions and has authored numerous peer-reviewed publications in regenerative medicine and translational research.

Prof. Dr. Amin Tamadon is a stem cell and regenerative medicine researcher with extensive experience in mesenchymal stem cells, extracellular vesicles, translational research, and preclinical therapeutic development. His work spans laboratory investigation, clinical translation, and systematic evidence synthesis. He has led nationally and internationally funded projects focusing on MSC-derived extracellular vesicles and regenerative therapies and has authored multiple peer-reviewed publications in stem cell biology and translational medicine.