Methods in Molecular Biology: Methods and Protocols

Barrientos, Antoni; Fontanesi, Flavia

This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species.  Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to study mitochondrial mRNAs that are translated on mitoribosomes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, The Mitoribosome: Methods and Protocols, aims to be a comprehensive guide for researchers in the field.

Details

Published by: Humana

Publication Date: 2023-05-12

Format: Hardcover

ISBN-13: 9781071631706

DOI: 10.1007/978-1-0716-3171-3

Dimensions: 254cm x178cm

Pages: 346