Methods in Molecular Biology: Methods and Protocols

Mysore, Kirankumar S.; Senthil-Kumar, Muthappa

This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Details

Published by: Humana

Publication Date: 2022-03-25

Format: Hardcover

ISBN-13: 9781071618745

DOI: 10.1007/978-1-0716-1875-2

Dimensions: 254cm x178cm

Pages: 328