MicroRNAs as Tools in Biopharmaceutical Production

Barron, Niall

Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells,  miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

Details

Published by: Springer

Publication Date: 2014-12-14

Format: Paperback

ISBN-13: 9789400795266

DOI: 10.1007/978-94-007-5128-6

Dimensions: 235cm x155cm

Pages: 126