Fluorescent dye design, synthesis, and biomedical application in one reference

Developing fluorescent dyes with the specificity, sensitivity, and photostability required for biological systems demands integrated knowledge spanning organic synthesis, photophysics, and biomedicine. Fluorescent Dyes: Synthesis and Biomedical Applications consolidates decades of progress into a single reference, covering fluorescence emission principles, rational dye design, and translational applications in sensing, labeling, bioimaging, medical diagnosis, and cancer treatment.

The book details fluorescent chemosensor design for selective recognition of bioactive small molecules in living systems, fluorescence-labeling agents targeting proteins and subcellular organelles, dyes engineered for high-resolution imaging, and theranostic agents combining diagnosis with therapy. Each topic addresses structure-property relationships governing performance under rigorous biological conditions, alongside future development directions for the field.

Readers will also find:

• New approaches for rational design of high-performance fluorescent dyes meeting the demanding requirements of in vivo biological applications
• Coverage of photodynamic therapy photosensitizers and their role in targeted cancer treatment strategies using fluorescence-guided approaches
• Analysis of fluorescence phenomena from fundamental photophysical principles through to practical probe construction and deployment in cellular environments
• Discussion of targeted nanodrug delivery systems incorporating fluorescent dyes for combined imaging and therapeutic functionality in living systems
• Evaluation of representative fluorescent dye classes developed over recent decades with detailed synthesis routes and characterization data

Designed for photochemists, cell biologists, pharmaceutical chemists, biochemists, organic chemists, analytical chemists, and sensor industry professionals, this reference provides the design principles, synthetic methods, and application frameworks needed to advance fluorescent dye research from molecular design through clinical translation.

Jiangli Fan, PhD, is Professor at the State Key Laboratory of Fine Chemicals, Dalian University of Technology, China. Her research focuses on small-molecule fluorescent probes for bioimaging and sensing, photosensitizer-based photodynamic therapy, and targeted nanodrug delivery. She serves on the editorial boards of Dyes and Pigments and Scientific Reports, and is a recipient of China's national outstanding youth fund.

Xiaojun Peng, PhD, is Director of the State Key Laboratory of Fine Chemicals at Dalian University of Technology, China, and editor-in-chief of Smart Molecules. Elected a member of the Chinese Academy of Sciences in 2017, his research covers dyes for fluorescent bioimaging, labeling, and digital printing. He has published more than 300 research papers with approximately 16,500 citations.