Proteomics in Practice

A Guide to Successful Experimental Design

Contributors: Westermeier, Reiner; Naven, Tom; Höpker, Hans-Rudolf

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

Book Details

• Publisher: Wiley-Blackwell
• Publication Date: 2008-05-05
• Format: Hardcover
• ISBN-13: 9783527319411
• Pages: 502