{"product_id":"9783319864211","title":"Springer Theses: High Throughput Sequencing of Multiple Transcripts from Single B Cells","description":"\u003ch1\u003eSpringer Theses: High Throughput Sequencing of Multiple Transcripts from Single B Cells\u003c\/h1\u003e \u003ch2\u003eDeKosky, Brandon\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening an entirely new window for antibody discovery and the investigation of adaptive immune responses to vaccines and diseases.\u003c\/p\u003e\u003cp\u003ePrevious methods for high-throughput immune repertoire sequencing have been unable to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. The author directly addresses these limitations by designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eThe techniques developed in this work have enabled comprehensive interrogation of human B-cell repertoires and have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Springer\u003c\/p\u003e \u003cp\u003ePublication Date: 2018-08-04\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9783319864211\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-3-319-58518-5\u003c\/p\u003e \u003cp\u003eDimensions: 235cm x155cm\u003c\/p\u003e \u003cp\u003ePages: 87\u003c\/p\u003e ","brand":"Springer International Publishing","offers":[{"title":"Default Title","offer_id":47394217492620,"sku":"9783319864211","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9783319864211.jpg?v=1775770197","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9783319864211","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}