Colorimetric detection is a widely used technique for detecting biological and non-biological analytes. This method generates a signal by developing color or discoloration of the assay. The application of nanomaterials can enhance the sensing signal at ultra-low concentrations of target molecules. Simple and rapid methodologies for the detection of biological and chemical targets are extremely important in the management of community health, disease diagnosis, and the environment. Colorimetric detection of biological and chemical moieties is particularly fascinating and attractive compared with numerous traditional methodologies due to its convenience, ease, and cost-effectiveness. The colorimetric signal is easy to assess with the naked eye without using any sophisticated instrument, making it a suitable assay for on-site detection. Generally, enzymes are used in colorimetric detection, which have some inherent limitations, such as complex preparation and purification costs, low operational stability, high sensitivity to environmental circumstances, and complications in reprocessing and reuse. Nowadays, nanomaterials-based colorimetric detection is very popular as it works under harsh circumstances, such as high pressures, high temperatures, extreme pH, and organic solvents. Research in this field is growing rapidly, significantly impacting different health and environmental aspects. However, there is no comprehensive information on the fabrication and application of these sensors in one place. This book will review the studies on the synthesis and modelling of nanomaterials that have led to breakthroughs and applications in colorimetric/ simple detection tools.

Dr. Ramesh Namdeo Pudake is an Assistant Professor at Amity University Uttar Pradesh, one of the top-ranked private universities in India. Dr. Pudake has a PhD in Crop Genetics and Breeding from China Agricultural University, Beijing, PRC. After his PhD, he is engaged in research in a range of organisms but with a focus on genomics and nanobiotechnology. He has also worked in the Department of Agronomy at Iowa State University, Ames, IA, USA on host-pathogen interaction and gene mapping. Currently, he is focusing on research on different applications of genomics and nanomaterials in agriculture. Dr. Pudake has published 50 research publications, 10 books, and 32 book chapters. He also has one scholarship award from the Chinese Government for pursuing his doctoral studies. He is an editorial board member and expert reviewer for several journals of repute.

Dr. Ravi Mani Tripathi is a Professor at the Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida, India. He earned his Ph.D. in Biotechnology from Jiwaji University, Gwalior, and completed his postdoctoral research at the School of Pharmacy, Sungkyunkwan University, South Korea. Dr. Tripathi is a recipient of the prestigious Teachers’ Associateship for Research Excellence (TARE) award conferred by the Science and Engineering Research Board (SERB), India. He was also honored with the Young Scientist Award at the International Scientist Award on Engineering, Science, and Medicine held in Visakhapatnam, India. With over 18 years of teaching and research experience in nanobiotechnology, his work focuses on the development of nanozyme-based colorimetric sensors for the detection of clinically important analytes. He has authored 60+ research articles and edited two books published by Elsevier.

Dr. Nesrin Horzum studied chemistry at the Pamukkale University, Türkiye in 2004. She did her M.Sc. and Ph.D. studies in Department of Chemistry at the Izmir Institute of Technology. She received her doctoral degree in 2013 from the Izmir Institute of Technology, with a thesis about the applications of electrospun nanofibers in filtration processes. During her Ph.D., she joined the Max Planck Institute for Polymer Research in August 2011 as a visiting scholar, investigating colloid electrospinning and surface functionalization of electrospun nanofibers. In 2014, she joined the group of Prof. Bo Liedberg at the Nanyang Technological University, where she worked at Centre for Biomimetic Sensor Science on paper based sensing platforms. She is now Professor in the Izmir Kâtip Çelebi University, Türkiye. She has authored about 50 scientific peer-reviewed publications, 2 books and 10 book chapters. Her current research interests include filtration and sensing applications of polymeric and hybrid nanofibers, as well as smartphone-based sensors for water quality monitoring and biomolecule detection.