Methods in Molecular Biology: Methods and Protocols

Jin, Hailing; Gassmann, Walter

RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.  This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets.  Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Details

Published by: Humana

Publication Date: 2012-05-21

Format: Hardcover

ISBN-13: 9781617798382

DOI: 10.1007/978-1-61779-839-9

Dimensions: 254cm x178cm

Pages: 231