{"product_id":"9781617792007","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eSchwartz, Philip H.; Wesselschmidt, Robin L.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eAlmost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments.  Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better\u003ci\u003e in vitro\u003c\/i\u003e human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in \u003ci\u003eHuman Pluripotent Stem Cells: Methods and Protocols\u003c\/i\u003e, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e            Authoritative and accessible, \u003ci\u003eHuman Pluripotent Stem Cells: Methods and Protocols\u003c\/i\u003e serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2011-08-09\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781617792007\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-61779-201-4\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 468\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542725185676,"sku":"9781617792007","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781617792007.jpg?v=1772842787","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781617792007","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}