Methods in Molecular Biology

Pflieger, Delphine; Rossier, Jean

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.

Details

Published by: Humana

Publication Date: 2010-11-19

Format: Paperback

ISBN-13: 9781617377723

DOI: 10.1007/978-1-59745-028-7

Dimensions: 235cm x155cm

Pages: 421