Methods in Molecular Biology

Lo, Y. M. Dennis

In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.

Details

Published by: Humana

Publication Date: 2010-12-01

Format: Paperback

ISBN-13: 9781617375118

DOI: 10.1385/159745074X

Dimensions: 229cm x152cm

Pages: 200