Methods in Molecular Biology: Methods and Applications

Casali, Nicola; Preston, Andrew

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

Details

Published by: Humana

Publication Date: 2003-07-03

Format: Hardcover

ISBN-13: 9781588291516

DOI: 10.1385/1592594093

Dimensions: 235cm x155cm

Pages: 316