Methods in Molecular Biology: Methods and Protocols

Scott, Mark G. H.; Laporte, Stéphane A.

This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in  MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson’s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Details

Published by: Humana

Publication Date: 2019-03-28

Format: Hardcover

ISBN-13: 9781493991570

DOI: 10.1007/978-1-4939-9158-7

Dimensions: 254cm x178cm

Pages: 409