{"product_id":"9781493987290","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eHacker, David L.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host;  however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\n\n\u003cp\u003eAuthoritative and convenient, \u003ci\u003eRecombinant Protein Expression in Mammalian Cells: Methods and Protocols\u003c\/i\u003e aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2018-09-22\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493987290\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-8730-6\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 311\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542915305612,"sku":"9781493987290","price":161.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493987290.jpg?v=1772847450","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493987290","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}