{"product_id":"9781493980598","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eJin, Tian; Hereld, Dale\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis second edition expands on the first edition with new chapters describing methods for studying\ncell movement, molecular components involved in chemotaxis, spatiotemporal\ndynamics of signaling components, and quantitative modeling, as well as several\nupdated chapters from the first edition. Various methods to investigate\ndirectional cell growth and movements are presented in Chapters 1-20. These\nchapters contains experimental procedures to visualize and measure migration\nbehaviors of different kinds of organisms, including chemotropism in the\nbudding yeast; cell growth and migration of D. discoideum; border cell\nmigration in Drosophila; chemotaxis of mouse and human neutrophils; and\nHIV-induced T cell chemotactic response. \u003ci\u003eChemotaxis: Methods and Protocols,\nSecond Edition\u003c\/i\u003e also contains microscopy procedures for studying breast cancer\ncell migration, tumor cell invasion in vivo, and axon guidance. The book concludes\nwith Chapters 21-27 describing methods that measure spatiotemporal dynamics of\nsignaling components involved in chemotaxis; introduce imaging techniques, such\nas TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models of\nexperimentally generated chemoattractant gradients. Written in the highly\nsuccessful Methods in Molecular Biology series format, chapters include\nintroductions to their respective topics, lists of the necessary materials and\nreagents, step-by-step, readily reproducible laboratory protocols, and tips on\ntroubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cbr\u003eCutting edge and thorough,\n\u003ci\u003eChemotaxis: Methods and Protocols, Second Edition\u003c\/i\u003e is a valuable resource for\nanyone who is interested in the diverse methodologies that are propelling\nchemotaxis research forward. \u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2018-05-26\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493980598\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-3480-5\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 425\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542913765516,"sku":"9781493980598","price":125.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493980598.jpg?v=1772847353","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493980598","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}