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This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Published by: Humana
Publication Date: 2019-09-13
Format: Hardcover
ISBN-13: 9781493976164
DOI: 10.1007/978-1-4939-7617-1
Dimensions: 254cm x178cm
Pages: 363