Methods in Molecular Biology: Methods and Protocols

Bornscheuer, Uwe T.; Höhne, Matthias

This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. 

The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com. 

Details

Published by: Humana

Publication Date: 2017-10-30

Format: Hardcover

ISBN-13: 9781493973644

DOI: 10.1007/978-1-4939-7366-8

Dimensions: 254cm x178cm

Pages: 350