Methods in Molecular Biology: Methods and Protocols

Bindewald, Eckart; Shapiro, Bruce A.

This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, RNA Nanostructures: Methods and Protocols is a valuable resource for the design and production of RNA nanostructures. Researchers and scientists sharing these detailed protocols is important for sustained progress in the field.

Details

Published by: Humana

Publication Date: 2017-07-21

Format: Hardcover

ISBN-13: 9781493971374

DOI: 10.1007/978-1-4939-7138-1

Dimensions: 254cm x178cm

Pages: 383