{"product_id":"9781493966479","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eNilles, Matthew L.; Condry, Danielle L. Jessen\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e Practical and comprehensive, \u003ci\u003eType 3 Secretion Systems: Methods and Protocols\u003c\/i\u003e is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.\u003cb\u003e\u003cbr\u003e \u003c\/b\u003e\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-11-12\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493966479\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-6649-3\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 224\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542872871052,"sku":"9781493966479","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493966479.jpg?v=1772846134","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493966479","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}