{"product_id":"9781493962983","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eSiomi, Mikiko C.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003ePIWI-interacting RNAs (piRNAs) are the third and most-recently discovered group of silencing-inducible small RNAs in animals. \u003ci\u003ePIWI-Interacting RNAs: Methods and Protocols\u003c\/i\u003e provides the most recent methods and protocols for studying piRNAs in the gonads of a wide range of species, as well as in any other organs where piRNAs may be detected. Comprehensive high-throughput sequencing analysis of piRNAs in embryos, testes and ovaries of \u003ci\u003eD. melanogaster,\u003c\/i\u003e as well as in mouse and rat testes, has raised the profile of piRNAs and thus further accelerated piRNA studies.\u003ci\u003e \u003c\/i\u003eIn addition to \u003ci\u003eC. elegans, \u003c\/i\u003eother model species such as \u003ci\u003eDrosophila melanogaster, Arabidopsis thaliana \u003c\/i\u003eand mice, along with\u003ci\u003e \u003c\/i\u003ecultured cell lines such as HeLa and \u003ci\u003eDrosophila\u003c\/i\u003e Schneider 2 (S2), and other such organisms have been used to address the fundamental questions of the biogenesis and functions of RNAi-triggering small non-coding RNAs. Written in the successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003eAuthoritative and easily accessible, \u003ci\u003ePIWI-Interacting RNAs: Methods and Protocols\u003c\/i\u003e seeks to serve both established researchers and newcomers to the field to progress towards the ultimate goal of understanding the mechanisms and actions of piRNAs.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-23\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493962983\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-62703-694-8\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 249\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542834761868,"sku":"9781493962983","price":107.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493962983.jpg?v=1772845168","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493962983","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}