{"product_id":"9781493961764","title":"Methods in Molecular Biology","description":"\u003ch1\u003eMethods in Molecular Biology\u003c\/h1\u003e \u003ch2\u003eMbikay, Majambu; Seidah, Nabil G.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eToday, activation endoproteolysis of secretory proteins is recognized as a fundamental biological mechanism of spatial and temporal regulation of protein activity as well as of diversification of protein functions.  In \u003ci\u003eProprotein Convertases\u003c\/i\u003e, experts in the field examine detailed methods involving proprotein convertases, the enzymes mediating this endoproteolysis, which reside within or cycle between the various compartments of the secretory pathway.  Providing a timely assessment of impact of activation\/inactivation endoproteolysis in the secretory pathway, the volume offers a broader perspective on the biochemistry of the PCSKs (proprotein convertases, subtilisin\/kexin-type) by exploring structural and functional analogies with bacterial subtilisin and on the enzymology of endoproteolysis itself by describing the involvement in the process of non-PCSK-type such as cathepsin L.  Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eMeticulous and up-to-date, \u003ci\u003eProprotein Convertases\u003c\/i\u003e represents an instructive and useful reference book for all scientists interested in endoproteolytic activation and\/or inactivation of secretory proproteins through limited proteolysis, for experts in the field and newcomers to it as well. \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-23\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493961764\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-61779-204-5\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 367\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542840266892,"sku":"9781493961764","price":143.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493961764.jpg?v=1772845549","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493961764","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}