{"product_id":"9781493958856","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eMacKenzie, Colin R.; Henrich, Birgit\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eSexually transmitted infections (STI) continue to be a major cause of morbidity and mortality, both in developed industrial countries as well as in the developing world. Human immunodeficiency virus infections and the ensuing opportunistic infections are a major drain on the human and financial resources of many countries in the developing world and even with the availability of effective treatment the epidemic is not yet contained. Screening in developing worlds is difficult and there is a need for simple reliable cheap diagnostic methods that can be performed at the point of care, on the same day and by staff with limited training. Molecular biological methods are very attractive for the diagnosis of STI since a well defined range of pathogens is responsible for the infection. \u003ci\u003eDiagnosis of Sexually Transmitted Diseases: Methods and Protocols\u003c\/i\u003e strives to cover the full range of molecular testing for STI. Chapters cover a variety of topics such as aspects of DNA extraction from small volume samples or difficult tissues, simple, nested or multiplex PCR, use of duplex primers or other modifications of primers and PCR conditions, sequence analysis for genotyping, denaturing gel analysis, microarrays using liquid beads or microspheres and silicon nanoparticle-enhanced microcantilever detection of DNA. Written in the successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eAuthoritative and easily accessible, \u003ci\u003eDiagnosis of Sexually Transmitted Diseases: Methods and Protocols\u003c\/i\u003e will be a valuable reference for the scientist looking to establish molecular methodologies for the diagnosis of STI tests intheir own lab.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-23\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493958856\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-61779-937-2\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 439\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542836924556,"sku":"9781493958856","price":143.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493958856.jpg?v=1772845309","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493958856","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}