{"product_id":"9781493958719","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eBill, Roslyn M.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eIn the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. \u003ci\u003eRecombinant Protein Production in Yeast: Methods and Protocols\u003c\/i\u003e examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eAuthoritative and practical,\u003ci\u003e Recombinant Protein Production in Yeast: Methods and Protocols,\u003c\/i\u003e seeks to aid scientists in adopting yeast as a protein production host.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-23\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493958719\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-61779-770-5\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 248\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542863892620,"sku":"9781493958719","price":107.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493958719.jpg?v=1772845749","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493958719","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}