{"product_id":"9781493949427","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eCastilho, Alexandra\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eConceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the \u003ci\u003eN\u003c\/i\u003e-glycans and glyco-profiling by mass spectrometry. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eAuthoritative and extensive, \u003ci\u003eGlyco-Engineering: Methods and Protocols\u003c\/i\u003e offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-10-09\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493949427\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-2760-9\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 439\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542830567564,"sku":"9781493949427","price":125.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493949427.jpg?v=1772844925","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493949427","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}