{"product_id":"9781493938087","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eKyba, Michael\u003c\/h2\u003e \u003cp\u003eThis volume focuses\non the cell biology and physiology of skeletal muscle regeneration. This Book\nis a collection of classic and cutting edge protocols optimized for mice, but\nin most cases adaptable to rat or other mammalian models, that will allow an\ninvestigator to develop and implement a research study on skeletal muscle\nregeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the\nprogenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by\neccentric contraction; volumetric muscle loss; single myofiber isolation and\nculture; satellite cell transplantation; muscle clearing for whole mount\nimmunostaining; luciferase tracking of muscle stem cells; mitochondrial\nand mitophagy flux analysis; in vivo assessment of muscle contractility;\nforce measurements on single isolated myofibers; and analysis of aerobic\nrespiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly\nsuccessful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries format,\nchapters include introductions to each respective topic, lists of the necessary\nmaterials and reagents, step-by-step, readily reproducible laboratory\nprotocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cbr\u003eCutting edge and\npractical, \u003ci\u003eSkeletal Muscle Regeneration in the Mouse: Methods and Protocols \u003c\/i\u003eis an essential laboratory reference for research in\nskeletal muscle growth, damage, repair, degeneration, and regenerative therapy\nin the mouse model system.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Springer\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-06\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493938087\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-3810-0\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 346\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542823981196,"sku":"9781493938087","price":143.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493938087.jpg?v=1772844540","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493938087","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}