{"product_id":"9781493933334","title":"Methods in Molecular Biology: Practice and Methods","description":"\u003ch1\u003eMethods in Molecular Biology: Practice and Methods\u003c\/h1\u003e \u003ch2\u003eMcCoy, Claire\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003ePractical and cutting-edge, \u003ci\u003eToll-Like Receptors: Methods and Protocols, Second Edition\u003c\/i\u003e is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.\u003cp\u003e\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-01-24\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493933334\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-3335-8\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 455\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542821851276,"sku":"9781493933334","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493933334.jpg?v=1772844404","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493933334","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}