{"product_id":"9781493929375","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eDoran, Pauline M.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; \u003ci\u003ein vivo\u003c\/i\u003e testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as ‘smart’ biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eAuthoritative and cutting-edge, \u003ci\u003eCartilage Tissue Engineering: Methods and Protocols\u003c\/i\u003e is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration. \u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2015-10-09\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493929375\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-2938-2\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 290\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542819360908,"sku":"9781493929375","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493929375.jpg?v=1772844306","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493929375","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}