{"product_id":"9781493927142","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eLehmann, Ulrich; Tost, Jörg\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThe primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003eComprehensive and adaptable, \u003ci\u003ePyrosequencing: Methods and Protocols, Second Edition\u003c\/i\u003e will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2015-06-24\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493927142\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-2715-9\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 414\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542817394828,"sku":"9781493927142","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493927142.jpg?v=1772844260","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781493927142","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}