Methods in Molecular Biology: Methods and Protocols

Beaujean, Nathalie; Jammes, Hélène; Jouneau, Alice

Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Details

Published by: Humana

Publication Date: 2014-10-07

Format: Hardcover

ISBN-13: 9781493915934

DOI: 10.1007/978-1-4939-1594-1

Dimensions: 254cm x178cm

Pages: 285