{"product_id":"9781489940384","title":"Methods in Molecular Biology","description":"\u003ch1\u003eMethods in Molecular Biology\u003c\/h1\u003e \u003ch2\u003eRapley, Ralph\u003c\/h2\u003e \u003cp\u003eAdvances in bioscience research usually arise as a result of the continu­ ing refinement of existing technologies. However, there are a number of occa­ sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu­ tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech­ nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac­ tion. This technique, first reported in 1985 by MuUis and his colleagues, pro­ vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2013-08-13\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781489940384\u003c\/p\u003e \u003cp\u003eDOI: 10.1385\/0896033449\u003c\/p\u003e \u003cp\u003eDimensions: 229cm x152cm\u003c\/p\u003e \u003cp\u003ePages: 221\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542807498892,"sku":"9781489940384","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781489940384.jpg?v=1772843659","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781489940384","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}