This new edition reflects the transformative developments in immunophenotyping research while maintaining a strong foundation in established methodologies essential for both research and clinical applications. Beginning with chapters involving fundamental immune cell populations, the book continues by exploring clinical applications in hematological malignancies and cellular therapies, as well as methodological advances and analytical frameworks essential for modern immunophenotyping. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Immunophenotyping: Methods and Protocols, Second Edition aims to provide researchers and clinicians with representative protocols spanning fundamental techniques to cutting-edge technologies.