This fully updated volume collects relevant and recent experimental approaches using atomic force microscopy (AFM) in biology and biomedical sciences. Beginning by addressing AFM imaging of biological samples and common practical considerations, the book continues by covering single molecule, molecular recognition approaches, protocols focused on membranes and bacteria, viruses and protein cages, and AFM nanomechanics and biomedical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding common pitfalls.
 
Authoritative and up-to-date, Atomic Force Microscopy: Methods and Protocols, Second Edition serves as a valuable reference for using AFM on biological and biomedical samples, AFM sample preparation, data acquisition and processing, as well as tips and tricks for optimizing AFM measurements.