{"product_id":"9781071655504","title":"Botrytis cinerea Methods and Protocols","description":"\u003ch3\u003eMethods in Molecular Biology\u003c\/h3\u003e\u003ch1\u003eBotrytis cinerea\u003c\/h1\u003e\u003ch2\u003eMethods and Protocols\u003c\/h2\u003e\u003ch3\u003eLuca Nerva | Walter Chitarra\u003c\/h3\u003e\u003cdiv\u003e\u003cb\u003eScience \/ Life Sciences \/ Mycology\u003c\/b\u003e\u003c\/div\u003e\u003cbr\u003e\u003cdiv\u003e\n\u003cp style=\"margin-top: 0in; background: white;\"\u003e\u003cspan style=\"color: black; mso-color-alt: windowtext;\"\u003eThis volume provides comprehensive protocols on Botrytis cinerea and the main methods used to study this important fungal species. Chapters detail cover classical approaches, including isolation, cultural techniques, sclerotia induction, storage, microscopy and protein localization, inoculation assays on fruits and vegetables, antifungal susceptibility testing, protoplast production and transfection, RNA interference, and identification of mycoviral infections. Additionally, the volume presents state-of-the-art molecular and omics-based approaches, including high-quality DNA and RNA extraction, molecular marker development, genome assembly, quantitative gene expression analysis, and integrative transcriptomic, proteomic, and metabolomic analyses.\u003c\/span\u003e\u003cspan style=\"color: #222222;\"\u003e Written for the highly successful \u003cem style=\"box-sizing: inherit; outline: 0px;\"\u003eMethods in Molecular Biology\u003c\/em\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.\u003c\/span\u003e\u003c\/p\u003e\r\n\u003cp style=\"background: white; box-sizing: inherit; margin-bottom: 2rem; overflow-wrap: break-word; word-break: break-word; outline: 0px; font-variant-ligatures: normal; font-variant-caps: normal; orphans: 2; text-align: start; widows: 2; -webkit-text-stroke-width: 0px; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; word-spacing: 0px;\"\u003e\u003cspan style=\"color: #222222;\"\u003eAuthoritative and comprehensive, \u003cem\u003eBotrytis cinerea: Methods and Protocols \u003c\/em\u003eaims to promote experimental rigor, facilitate cross-disciplinary collaboration, and stimulate innovative research directions in \u003cem\u003eBotrytis\u003c\/em\u003e biology.\u003c\/span\u003e\u003c\/p\u003e\r\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003cbr\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublication Date: \u003c\/td\u003e\n\u003ctd\u003e06 November 2026\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublisher: \u003c\/td\u003e\n\u003ctd\u003eSpringer US\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImprint: \u003c\/td\u003e\n\u003ctd\u003eHumana\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eISBN-13: \u003c\/td\u003e\n\u003ctd\u003e9781071655504\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat: \u003c\/td\u003e\n\u003ctd\u003eHardback\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Springer US","offers":[{"title":"Default Title","offer_id":50450083840140,"sku":"9781071655504","price":179.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071655504.jpg?v=1780593644","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071655504","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}