{"product_id":"9781071653159","title":"Bacterial Multidrug Exporters Methods and Protocols","description":"\u003ch3\u003eMethods in Molecular Biology\u003c\/h3\u003e\u003ch1\u003eBacterial Multidrug Exporters\u003c\/h1\u003e\u003ch2\u003eMethods and Protocols\u003c\/h2\u003e\u003ch3\u003eKunihiko Nishino | Mikio Tanabe\u003c\/h3\u003e\u003cdiv\u003e\u003cb\u003eScience \/ Life Sciences \/ Biochemistry\u003c\/b\u003e\u003c\/div\u003e\u003cbr\u003e\u003cdiv\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\" style=\"mso-ansi-language: EN-CA;\"\u003eThis second edition volume expands on the previous edition with updates on the latest advancements in the field that span from atomic-resolution structural analysis to whole-organism functional studies. The chapters in this book are organized into four parts. Part One: Structural Analysis Methods\u003cem\u003e \u003c\/em\u003epresents cutting-edge approaches for determining high-resolution structures of multidrug exporters, and covers the full spectrum of structural biology techniques, from solution NMR spectroscopy for studying PfMATE to single-particle cryo-EM analysis of TolC. Part Two: Biochemical Analysis and Reconstitution focuses on functional characterization of multidrug exports and looks at methods such as site-specific fluorescent labeling of AcrB and analytical ultracentrifugation for studying oligomeric states. Part Three: Computational and Simulation Approaches showcases the power of in silico methods in multidrug exporter research such as molecular dynamics simulations of conformational changes in MdfA. Part Four: Genetic Tools and Functional Studies\u003cem\u003e \u003c\/em\u003epresents a diverse array of methods for investigating multidrug exporters in their biological context. This section explores high-throughput gene mapping using TraDISort, evaluation of efflux pump inhibitors through growth curve analysis, and cloning and characterization protocols. \u003c\/span\u003eWritten in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cspan lang=\"EN-CA\" style=\"mso-ansi-language: EN-CA;\"\u003e\u003cbr\u003e\u003c\/span\u003eComprehensive and cutting-edge, \u003cem\u003eBacterial Multidrug Exporters: Methods and Protocols, Second Edition \u003c\/em\u003eis a valuable resource for novel researchers entering this field and established investigators seeking to expand their methodological range.\u003c\/p\u003e\u003c\/div\u003e\u003cbr\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublication Date: \u003c\/td\u003e\n\u003ctd\u003e02 August 2026\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublisher: \u003c\/td\u003e\n\u003ctd\u003eSpringer US\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImprint: \u003c\/td\u003e\n\u003ctd\u003eHumana\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eISBN-13: \u003c\/td\u003e\n\u003ctd\u003e9781071653159\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat: \u003c\/td\u003e\n\u003ctd\u003eHardback\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePage Count: \u003c\/td\u003e\n\u003ctd\u003e275\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Springer US","offers":[{"title":"Default Title","offer_id":46412069437580,"sku":"9781071653159","price":197.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071653159.jpg?v=1781057428","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071653159","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}