{"product_id":"9781071651933","title":"Collagen Methods and Protocols","description":"\u003ch3\u003eMethods in Molecular Biology\u003c\/h3\u003e\u003ch1\u003eCollagen\u003c\/h1\u003e\u003ch2\u003eMethods and Protocols\u003c\/h2\u003e\u003ch3\u003eIrit Sagi | Nikolaos A. Afratis\u003c\/h3\u003e\u003cdiv\u003e\u003cb\u003eScience \/ Life Sciences \/ Molecular Biology\u003c\/b\u003e\u003c\/div\u003e\u003cbr\u003e\u003cdiv\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\" style=\"mso-ansi-language: EN-CA;\"\u003eThis second edition volume expands on the previous edition with a discussion of the latest methods used to \u003c\/span\u003eexplore collagen biology from molecular synthesis and folding to biomechanical analysis and tissue engineering applications. The chapters in this book are organized into four parts: Part One introduces strategies for manipulating collagen at the molecular level, including gene targeting, mutagenesis, and functional assays. Part Two focuses on cellular and biochemical aspects of collagen biology, featuring protocols to study the effects of hypoxia, assess triple helix stability, and investigate ECM-rich environments using proteomics and mass spectrometry. Part Three covers structural and imaging techniques such as atomic force microscopy and advanced visualization of collagen suprastructures, and Part Four looks at translational and biomedical research, providing protocols for constructing 3D ECM scaffolds, engineering collagen-based biomaterials, and modeling tumor microenvironments. Written in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cspan lang=\"EN-CA\" style=\"mso-ansi-language: EN-CA;\"\u003e\u003cbr\u003e\u003c\/span\u003eCutting-edge and comprehensive, \u003cem\u003eCollagen: Methods and Protocols, Second Edition \u003c\/em\u003eis a valuable resource for researchers in cell biology, biochemistry, tissue engineering, and translational medicine, and will support the development of novel strategies to explore and manipulate the power of collagen.\u003c\/p\u003e\u003c\/div\u003e\u003cbr\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublication Date: \u003c\/td\u003e\n\u003ctd\u003e21 July 2026\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublisher: \u003c\/td\u003e\n\u003ctd\u003eSpringer US\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImprint: \u003c\/td\u003e\n\u003ctd\u003eHumana\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eISBN-13: \u003c\/td\u003e\n\u003ctd\u003e9781071651933\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat: \u003c\/td\u003e\n\u003ctd\u003eHardback\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePage Count: \u003c\/td\u003e\n\u003ctd\u003e280\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Springer US","offers":[{"title":"Default Title","offer_id":45382551470220,"sku":"9781071651933","price":179.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071651933.jpg?v=1780593593","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071651933","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}