{"product_id":"9781071650554","title":"Macrophage Migration Inhibitory Factor Methods and Protocols","description":"\u003ch3\u003eMethods in Molecular Biology\u003c\/h3\u003e\u003ch1\u003eMacrophage Migration Inhibitory Factor\u003c\/h1\u003e\u003ch2\u003eMethods and Protocols\u003c\/h2\u003e\u003ch3\u003eJames Harris | Timothy W. Muusse\u003c\/h3\u003e\u003cdiv\u003e\u003cb\u003eScience \/ Life Sciences \/ Biochemistry\u003c\/b\u003e\u003c\/div\u003e\u003cbr\u003e\u003cdiv\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\" style=\"mso-ascii-font-family: Aptos; mso-fareast-font-family: Aptos; mso-hansi-font-family: Aptos; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-CA;\"\u003eThis second edition volume expands on the previous edition with new protocols that offer biochemical, biophysical, and biological assays for studying macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (D-DT). The chapters in this book cover topics such as generation of MIF peptides via solid phase peptide synthesis; fragment screening of MIF by surface plasmon resonance; MIF NMR chemical shift perturbation mapping; methods to determine the role of MIF in cilia biogenesis; and immunofluorescent quantification of MIF in larval zebrafish tailfin transfection. \u003c\/span\u003e\u003cspan style=\"mso-ascii-font-family: Aptos; mso-fareast-font-family: Aptos; mso-hansi-font-family: Aptos; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US;\"\u003eWritten in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003c\/span\u003e\u003cspan lang=\"EN-CA\" style=\"mso-ascii-font-family: Aptos; mso-fareast-font-family: Aptos; mso-hansi-font-family: Aptos; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-CA;\"\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan style=\"mso-ascii-font-family: Aptos; mso-fareast-font-family: Aptos; mso-hansi-font-family: Aptos; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US;\"\u003eCutting-edge and comprehensive, \u003cem\u003eMacrophage Migration Inhibitory Factor: Methods and Protocols, Second Edition \u003c\/em\u003eis a valuable resource for any researcher hoping to contribute to the ever expanding world of MIF biology.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003cbr\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublication Date: \u003c\/td\u003e\n\u003ctd\u003e15 May 2026\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublisher: \u003c\/td\u003e\n\u003ctd\u003eSpringer US\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImprint: \u003c\/td\u003e\n\u003ctd\u003eHumana\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eISBN-13: \u003c\/td\u003e\n\u003ctd\u003e9781071650554\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat: \u003c\/td\u003e\n\u003ctd\u003eHardback\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePage Count: \u003c\/td\u003e\n\u003ctd\u003e296\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44499610337420,"sku":"9781071650554","price":197.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071650554.jpg?v=1781057403","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071650554","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}