{"product_id":"9781071645499","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eDeweese, Joseph\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-size: 12.0pt; line-height: 107%; font-family: 'Times New Roman',serif;\"\u003eThis volume is intended to provide both fundamental methods for long-standing assays as well as recent advances in the field toward newer pathways. Chapters guide readers through methods tailored to studying prokaryotic topoisomerases including fundamental protocols for purification and basic characterization of prokaryotic enzymes, enzymology examining DNA cleavage, relaxation, decatenation, and binding to more complex assays dissecting cellular function and interactions, and \u003c\/span\u003e\u003cspan lang=\"EN\" style=\"font-size: 12.0pt; line-height: 107%; font-family: 'Times New Roman',serif; mso-ansi-language: EN;\"\u003ebioinformatics protocol for examining protein sequence interdependencies with application across many different proteins beyond topoisomerases. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-size: 12.0pt; line-height: 107%; font-family: 'Times New Roman',serif;\"\u003e \u003c\/span\u003e\u003cspan style=\"font-size: 12.0pt; line-height: 107%; font-family: 'Times New Roman',serif;\"\u003eAuthoritative and cutting-edge, \u003cem\u003eTopoisomerases: Methods and Protocols\u003c\/em\u003e\u003cstrong\u003e \u003c\/strong\u003eas a resource both to new investigators and to long-standing researchers in the field of topoisomerases.\u003c\/span\u003e\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2025-05-16\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071645499\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-4550-5\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 234\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":45228019449996,"sku":"9781071645499","price":197.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071645499.jpg?v=1772892379","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071645499","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}