{"product_id":"9781071643136","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eFratti, Rutilio\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis second edition details new and updated methods on \u003cem\u003es\u003c\/em\u003eoluble \u003cem\u003eN\u003c\/em\u003e-ethylmaleimide-sensitive factor \u003cem\u003ea\u003c\/em\u003ettachment protein \u003cem\u003ere\u003c\/em\u003eceptors( SNAREs) and their function are examined in the laboratory. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF\/Sec18 and a-SNAP\/Sec17, examining the structures and interactions of SNAREs, measuring the interactions of SNAREs, interactions of SNAREs, and post-translational modifications of SNAREs and how they affect function. Written in the format of the highly successful \u003cem\u003eMethods in Molecular Biology \u003c\/em\u003eseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eAuthoritative and cutting-edge, \u003cem\u003eSNAREs: Methods and Protocols, Second Edition \u003c\/em\u003eaims to provide detailed methods so that novice as well as experienced researchers can explore the mechanisms of SNARE-mediated membrane fusion.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2025-01-14\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071643136\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-4314-3\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 299\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":45659302199436,"sku":"9781071643136","price":224.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071643136.jpg?v=1775754039","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071643136","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}