{"product_id":"9781071642221","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eSchindler, Daniel\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume provides an overview of the latest protocols that cover the full range of \u003cem\u003ein silico\u003c\/em\u003e, \u003cem\u003ein vitro\u003c\/em\u003e, and \u003cem\u003ein vivo\u003c\/em\u003e approaches to help researchers plan, design, and perform Golden Gate cloning experiments. The chapters in this book cover topics such as Golden Gate cloning of standardized parts; modular DNA construct design for high-throughput Golden Gate Assembly; Golden Gate cloning of Synthetic CRISPR RNA spacer sequences; Golden Gate cloning in Actinobacteria; and YeastFab cloning of toxic genes and protein expression optimization in yeast. Written in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\n\u003cbr\u003e\nCutting-edge and thorough, \u003cem\u003eGolden Gate Cloning: Methods and Protocols \u003c\/em\u003eis a valuable resource for all researchers interested in learning more about this important and developing field.\u003cbr\u003e\n\u003cbr\u003e\nChapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2025-10-05\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071642221\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-4220-7\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 503\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":49471502844044,"sku":"9781071642221","price":125.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071642221.jpg?v=1777985564","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071642221","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}