{"product_id":"9781071633724","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eBhakat, Kishor K.; Hazra, Tapas K.\u003c\/h2\u003e \u003cp\u003eThis detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway \u003ci\u003ein vitro\u003c\/i\u003e and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003c\/p\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and practical, \u003ci\u003eBase Excision Repair Pathway: Methods and Protocols\u003c\/i\u003e serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions \u003ci\u003ein vitro\u003c\/i\u003e and in cell by the distinct set of proteins in the BER pathway.\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2023-08-14\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071633724\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-3373-1\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 262\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44521693216908,"sku":"9781071633724","price":197.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071633724.jpg?v=1772891944","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071633724","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}