{"product_id":"9781071631669","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eLodhi, Irfan J.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using \u003ci\u003ein vitro\u003c\/i\u003e cell culture and animal models.  Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism in mice; and techniques for AAV-mediated gene delivery, transplantation of adipose tissue, isolation of adipose tissue immune cells and extracellular vesicles, and mass spectrometry-based profiling of brown fat lipids.  Written in the format of the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eAuthoritative and cutting-edge,\u003ci\u003eThermogenic Fat: Methods and Protocols\u003c\/i\u003e aims to be comprehensive guide for researchers in the field. \u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2023-04-20\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071631669\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-3167-6\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 253\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44521691480204,"sku":"9781071631669","price":161.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071631669.jpg?v=1772891831","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071631669","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}