{"product_id":"9781071628614","title":"Methods in Molecular Biology","description":"\u003ch1\u003eMethods in Molecular Biology\u003c\/h1\u003e \u003ch2\u003eRodriguez, Manuel S; Barrio, Rosa\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume details novel and popular approaches to decipher the \u003ci\u003eUbiquitin Code\u003c\/i\u003e. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation \u003ci\u003ein vitro, a \u003c\/i\u003eRNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8\u003csup\u003eR74K \u003c\/sup\u003emutant, \u003ci\u003ein vivo\u003c\/i\u003e identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3\/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others. Written in the successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eAuthoritative and cutting-edge, \u003ci\u003eThe Ubiquitin Code \u003c\/i\u003eaims\u003ci\u003e \u003c\/i\u003eto to be a useful practical guide to researches to help further their study in this field. \u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2023-11-30\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071628614\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-2859-1\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 245\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44806645547148,"sku":"9781071628614","price":125.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071628614_8548af73-7092-4d96-83e6-5f2b0eda1a3d.jpg?v=1772891660","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071628614","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}