{"product_id":"9781071625569","title":"Hepatocytes: Methods and Protocols","description":"\u003ch1\u003eHepatocytes: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eTanimizu, Naoki\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume details experimental protocols to study structure and function of hepatocytes \u003ci\u003ein vivo\u003c\/i\u003e and \u003ci\u003ein vitro\u003c\/i\u003e, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepatoblastoma and identifying hepatocellular carcinoma subtypes. Written in the format of the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eAuthoritative and cutting-edge, \u003ci\u003eHepatocytes: Methods and Protocols \u003c\/i\u003eaims to provide techniques to further understand molecular mechanisms involved in the regulating hepatocyte functions and underlying liver diseases.\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2022-09-21\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071625569\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-2557-6\u003c\/p\u003e \u003cp\u003eDimensions: 254.0cm x178.0cm\u003c\/p\u003e \u003cp\u003ePages: 281.0\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44806643089548,"sku":"9781071625569","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071625569.jpg?v=1763577324","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071625569","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}