{"product_id":"9781071624012","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eMapelli, Valeria; Bettiga, Maurizio\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cdiv\u003eThis second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of \u003ci\u003eSaccaromyces cerevisiae\u003c\/i\u003e and \u003ci\u003eKomagataella\u003c\/i\u003e spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of \u003ci\u003eS. cerevisiae, P. pastoris,\u003c\/i\u003e and \u003ci\u003eZ. bailii\u003c\/i\u003e; the use of yeast in CRISPR\/Cas9 technology; tools to study metabolic pathway in \u003ci\u003eYarrowia lypolitica\u003c\/i\u003e; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003eCutting-edge and authoritative, Y\u003ci\u003eeast Metabolic Engineering: Methods and Protocols, Second Edition\u003c\/i\u003e is a valuable resource for researchers and scientists interested in learning more about this important and developing field.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2022-07-04\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071624012\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-2399-2\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 295\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":46543564472460,"sku":"9781071624012","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071624012.jpg?v=1772849815","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071624012","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}