{"product_id":"9781071623701","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eMus-Veteau, Isabelle\u003c\/h2\u003e \u003cp\u003eThis detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using \u003ci\u003eE. coli\u003c\/i\u003e as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. \u003c\/p\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and up-to-date, \u003ci\u003eHeterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition\u003c\/i\u003e serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2023-07-02\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071623701\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-2368-8\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 467\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":45382548226188,"sku":"9781071623701","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071623701.jpg?v=1772849802","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071623701","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}