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This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Published by: Humana
Publication Date: 2022-06-24
Format: Hardcover
ISBN-13: 9781071622889
DOI: 10.1007/978-1-0716-2289-6
Dimensions: 254cm x178cm
Pages: 357