{"product_id":"9781071621516","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eCurrin, Andrew; Swainston, Neil\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cbr\u003e\u003cbr\u003eCutting-edge and comprehensive, \u003ci\u003eDirected Evolution: Methods and Protocols\u003c\/i\u003e is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2022-06-22\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071621516\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-2152-3\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 278\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":45988220567692,"sku":"9781071621516","price":224.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071621516.jpg?v=1775753652","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071621516","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}