Methods in Molecular Biology: Methods and Protocols

Klemenčič, Marina; Stael, Simon; Huesgen, Pitter F.

This volume presents current methods to detect and measure the activity of proteolytic enzymes in organisms ranging from unicellular algae to flowering plants. Chapters detail in vitro production, characterization of plant proteases, tools for in vivo modifications, proteomic approaches for identification of substrates, inhibitors and interacting partners, and the function of plant proteases in plant programmed cell death. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Plant Proteases: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 

Details

Published by: Humana

Publication Date: 2022-05-19

Format: Hardcover

ISBN-13: 9781071620786

DOI: 10.1007/978-1-0716-2079-3

Dimensions: 254cm x178cm

Pages: 299